PBS Unit 1 - Review Sheet
Whats Covered in this Review Sheet
- Processing a Crime Scene
- Fingerprints & Hair
- Blood Testing
- Glastier Equation
- Standard Curves & Correlation
- DNA Structure
- Polymerase Chain Reaction
- Restriction Enzymes
- DNA Gel Electrophoresis
- Manner of Death & Cause of Death
- Body Systems
- HIPAA
Processing a Crime Scene (Activity 1.1.1 - Investigating the Scene)
Sketch
- Record
- Position of Victim
- Evidence
- Layout
- Diagram
Legend
Scale
Key
Photograph
- Close/ High Quality
- Include Ruler
Interview
- Questions
- What happened?
- Was it a crime?
- How did it happen?
- Develop hypothesis.
Collect Evidence
- Link evidence to tell a story
- Analyze evidence
- Avoid contaimination
Examine
- Search Method
- Mark Evidence
Evidence (Activity 1.1.4 - The Evidence)
Fingerprint Patterns
Loop Whorl Arch
Hair Structure
- Cuticle – Outermost layer; covers/ protects the hair
- Cortex – Between cuticle and medulla; contains pigment (melanin) for hair color; thickest layer.
- Medulla – Innermost layer; soft/ fragile.
Blood Testing (Activity 1.1.4 - The Evidence)
Blood Type A
- Antigens: A
- Antibodies: B
Blood Type B
- Antigens: B
- Antibodies: A
Blood Type AB
- Antigens: AB
- Antibodies: none
Blood Type O
- Antigens: none
- Antibodies: AB
Glastier Equation (Activity 1.1.5 - Time of Death)
Standard Curve & Correlation (Activity 1.1.6 - Blood Spatter)
Linear Regression – Best Fit Line
- A linear regression – or best-fit line – attempts to represent data points in a scatter plot as a representative trend.
- Equation: $y = mx + b$
- A linear regression – or best-fit line – attempts to represent data points in a scatter plot as a representative trend.
$y$ = y variable
$m$ = slope
$x$ = x variable
$b$ = y intercept
Nucleotides (Activity 1.2.1 - What is DNA?)
Structure: A phosphate group, deoxyribose sugar and a nitrogen base are all the components that make up a nucleotide. The organization of each structural component can be seen to the right.
Function: Nucleotides are marcomolecule-monomers that can be brought together to create a DNA polymer; they are the ‘building blocks’ of DNA.
Nitrogen Bases (Activity 1.2.1 - What is DNA?)
Purines
- Double-ring structure
- Guanine base pairs with Cytosine
- • They share 3 hydrogen bonds
- Adenine base pairs with Thymine
- • They share 2 hydrogen bonds
Pyrimidines
- Single-ring structure
- Cytosine base pairs with Guanine
- • They share 3 hydrogen bonds
- • They share 3 hydrogen bonds
- Thymine base pairs with Adenine
- • They share 2 hydrogen bonds
Polymerase Chain Reaction (Activity 1.2.3 - DNA Analysis)
Step 1 – Denaturation
- Raise the temperature.
- As a result, the hydrogen bonds in the DNA double strand break and the molecule separates into two separate DNA single strands. (Excuse the alliteration!)
Step 2 – Annealing
- Cool the temperature down a bit.
- This will allow primers to attach to complimentary sequences on each of the single stranded DNA.
Step 3 – Elongation
- Slightly raise the temperature
- Taq polymerase will interact with the DNA-primer complex and begin to add nucleotides to the 3′ end of the primer. These nucleotides will be complimentary to whatever nucleotides are on the DNA strand.
Restriction Endonucleases (Activity 1.2.3 - DNA Analysis)
Origin: Restriction enzymes are produced by bacteria to protect the bacterium from foreign, viral DNA.
Function: They recognize a short, specific nucleotide sequences and separate the DNA strands at precise locations.
Application: Scientists have isolated restriction enzymes from bacteria and used them for techniques like genetic cloning and gel electrophoresis.
Gel Electrophoresis (Activity 1.2.3 - DNA Analysis)
Purpose: DNA gel electrophoresis attempts to separate DNA fragments according to the size of the fragments.
Function: The DNA sample is loaded into the wells of the gel and a current is applied to the electrophoresis chamber which also contains a salt water buffer. The DNA is moves towards the positive electrode in response to an applied current. Larger DNA fragments move slower through the agarose molecules in the gel.
Application: Gel electrophoresis can be used to analyze and compare the RFLPs of an unknown genetic sample to known genetic samples. For example, DNA evidence at a crime scene can be compared to a series of potential suspects.
Autopsy (Activity 1.3.1 - Autopsy)
Manner of Death
- Homicide
- Suicide
- Accidental
- Natural
- Undetermined
- Pending
Cause of Death – What specifically caused the death?
- Physician; Medical Doctor
- Preform autopsies
- Determine manner & cause of death
- Appointed official
- Investigates the death.
- Determine manner & cause of death
Body Systems (Activity 1.3.1 - Autopsy)
Integumentary System
- Structures – skin; hair; nails
- Function – protection
Nervous System
- Structure – brain; spinal cord; nerves
- Function – communication
Digestive System
- Structure – oral cavity; esophagus; stomach; intestines
- Function – water and energy absorption; solid waste
Respiratory System
- Structure – nasal passage; trachea; lungs; bronchi; aveoli
- Function – gas exchange
Urinary System
- Structure – kidney; ureter; bladder; urethra
- Function – water absorption; liquid waste
Endocrine System
- Structure – pituitary gland; adrenal gland; ovaries; testes
- Function – hormone regulation; communication
Lymphatic & Immune System
- Structure – lymph nodes; thymus; spleen; bone marrow
- Function – filtration of toxins and waste; immunity
Skeletal System
- Structure – bones; joints
- Function – structure; protection
Muscular System
- Structure – skeletal muscle; smooth muscle; cardiac muscle
- Function – movement; heat
Cardiovascular System
- Structure – heart; arteries; veins; capillaries
- Function – circulate blood; gas exchange
Health Insurance Portability & Accountability Act (Activity 1.3.2 - Confidentiality)
